Biotin-labelled antigen screening test for toxoplasma IgM antibody.
نویسندگان
چکیده
منابع مشابه
Preparation of Biotin-labelled Toxoplasma Antigen
A method for the simple preparation ofbiotin-labelled toxoplasma antigen was used with avidin peroxidase in an IgM-capture enzyme linked immunosorbent assay (BAM-ELISA). Although the overall predictive value ofa positive result was only 38%, its low-cost and 100% sensitivity makes it a very suitable screening test. Positive results can be confirmed by an alternative assay, thus providing a more...
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A new serologic test for antibodies to Toxoplasma is described, which is based upon inhibition of specific staining with fluorescent antibody. In performing the test, a mixture of the test serum and known fluorescein-labelled antiserum is added to a dried smear of toxoplasms for 1 hour at 37 degrees C. The smear is then rinsed and examined with a fluorescence microscope. Reduction in the bright...
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Congenital toxoplasmosis is associated with important morbidity and mortality. Since vertical transmission of Toxoplasma gondii can occur in acute cases, antenatal screening for recent infections is vital. Accurate determination of acute toxoplasmosis requires a combination of immunoassays, usually not routinely applied for screening purposes. This study evaluated the anti-T. gondii (IgG+IgM)/I...
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Antiphospholipid antibodies, including anticardiolipin antibodies, are frequently detected in sera from patients with systemic lupus erythematosus (1). Numerous reports have associated these autoantibodies with various venous and arterial thrombotic disorders, including cerebral infarction (2), deep venous thrombosis (3), thrombocytopenia (1), pulmonary embolism (4), and recurrent fetal loss wi...
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Background: Toxoplasmosis is a zoonotic disease with high seroprevalence worldwide. Several immunological methods have been described for diagnosis of toxoplasmosis. Objective: To determine the parasitemia period in patients infected with toxoplasma using PCR and comparing serological data with molecular results. Methods: 154 serum samples from patients with toxoplasmosis were examined. Presenc...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1989
ISSN: 0021-9746
DOI: 10.1136/jcp.42.2.206